Promotional price valid on web orders only. Your contract pricing may differ. Interested in signing up for a dedicated account number?
Learn More

Applied Biosystems™ resDNASEQ™ Quantitative E. coli DNA Kits
SDP

This quantitative PCR (qPCR)-based kit, part of the resDNASEQ™ E. coli Residual DNA Quantitation System, enables highly sensitive quantitation of host cell DNA from E. coli expression systems, typically in less than 4 hours.

$3500.00 - $4035.00

Specifications

Detection Method Primer-probe
Format Kit
PCR Method qPCR
Host Cell E.coli
Label or Dye FAM
View More Specs

Products 2
Catalog Number Mfr. No. Includes Price Quantity & Availability  
Catalog Number Mfr. No. Includes Price Quantity & Availability  
A4458435
SDP
View Documents
Applied Biosystems™
4458435
Assay Kit only
Each for $3,500.00
Add to Cart
 
A4460366
SDP
View Documents
Applied Biosystems™
4460366
Assay Kit and Sample Preparation
Each for $4,035.00
Add to Cart
 
Description

Description

This quantitative PCR (qPCR)-based kit, part of the resDNASEQ™ E. coli Residual DNA Quantitation System, enables highly sensitive quantitation of host cell DNA from E. coli expression systems, typically in less than 4 hours. The kit is part of an integrated system that includes sample preparation, TaqMan™ Assay and master mix, standard DNA, instrument, and software. The system overcomes the limitations of traditional methods by combining high-recovery PrepSEQ™ sample preparation and TaqMan™ Assay-based quantitation of residual DNA.

Features of the resDNASEQ Quantitative E. coli DNA Kit include:
• Highly sensitive quantitation using proven TaqMan™ real-time qPCR technology
• Manual and automated sample preparation, optimized for quantitative recovery from complex sample matrices (Table 1)
• Consistent performance across the expected range of DNA fragment sizes
• Integrated system from sample to results with sample preparation, master mix, TaqMan™ primer/probe mix, and genomic DNA standard

This kit is available with or without sample preparation reagents in the form of a PrepSEQ™ Residual DNA Sample Preparation Kit, which is optimized for highly efficient residual DNA recovery from complex mixtures of proteins, buffers, and salts.

Rapid, Sensitive Detection of E.Coli DNA
The resDNASEQ™ E. coli Residual DNA Quantitation System is a quantitative PCR (qPCR)-based system for the detection of host cell DNA from E. coli, an expression system commonly used for the production of recombinant proteins. Reliable and rapid, the system enables sensitive (LOQ = 15 pg DNA/mL test sample) and specific quantitation of E. coli DNA, typically in less than 4 hours. This performance helps ensure a high degree of confidence in quantitation data obtained from a broad range of sample types—from in-process samples to bulk drug substances—whether a sample contains high molecular weight or sheared DNA.

Table 1. DNA recovery using manual PrepSEQ™ sample preparation protocol.
Assay performance data using 10 pg E. coli genomic DNA spike per sample, 3 analysts, and 9 test samples.

  50 mg/mL protein sample 
  Mean Recovery Mean %CV
E. coli83%5.04%


Specifications

Specifications

Primer-probe
qPCR
FAM
100 Reactions
Kit
E.coli
resDNASEQ™
SDS
Documents

Documents

Product Certifications

For Research Use Only. Not for use in diagnostic procedures.

Provide Content Correction

Your input is important to us. Please complete this form to provide feedback related to the content on this product.

Product Title

By clicking Submit, you acknowledge that you may be contacted by Fisher Scientific in regards to the feedback you have provided in this form. We will not share your information for any other purposes. All contact information provided shall also be maintained in accordance with our Privacy Policy.